These machines have two types of reagents - P2 and P3 that differ in the number of clusters generated.
There is no difference in the reagents if you run Paired-end or Single-end.
That means that for example in P3 200 kit, you can run this as 200bp Single-end or 100bp Paired-end.
Expecting to have around 1100 MIO single-reads or read-pairs respectively.
Important: For pair-end sequencing, it is necessary to have dual index setup because of index hopping ( more on that here. )
Below you can find a table, comparing the NS2000 with NS500:
| Reagent Type | Reagent Kit size | Maximum Number Of Bases | |
|---|---|---|---|
| NextSeq 500 | Mid (130 MIO clusters) or High (400 MIO clusters) Output |
75 | 92 |
| 150 | 168 | ||
| 300 | 318 | ||
| NextSeq 2000 | P2 (400 MIO clusters) | 100 | 138 |
| 200 | 238 | ||
| 300 | 327 | ||
| P3 (1100 MIO clusters) | 50 | 88 | |
| 100 | 138 | ||
| 200 | 238 | ||
| 300 | 327 | ||
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GeneCore is open.
We are receiving external samples.
If you are sending ready-to-run libraries, please note they need not be sent on dry ice. All samples should be sent in 1.5 mL low-binding tubes.
If you have questions, we kindly ask you to contact solexateam@embl.de before sending samples to us.
Please take care and stay safe.
Your GeneCore team